Deliverable 1:Tabula Sapiens Dataset Overview

• Link to Google Colaboratory (Please install Anaconda to view ipynb file on Google Colab/ Jupyter Notebook locally)
• Python File for the above notebook
• View Slides - Overview of dataset

Implementation Steps:

1. Download and install Python, Anaconda and Jupyer Notebook.
2. Download Tabula Sapiens - Heart Dataset from CZ Biohub website.
3. Install anndata, scanpy, numpy, pandas, matplotlib, seaborn python packages.
4. Execute the code snippets step by step as mentioned in Google Colab(ipynb) file.

Results:

1. The data is in h5ad format which is specific to R language for single cell RNA sequencing.


2. The data present in Heart Dataset contains 11,505 cells and 56,604 genes.


3. The attributes of the Heart data


4. The names of first 10 genes are shown below.


5. Performed Dimensionality Reduction using UMAP (Uniform Manifold Approximation and Projection)


6. Performed Dimensionality Reduction using PCA (Principle Component Analysis)